Efficient RNA interference (RNAi) in Mamallian Cells
نویسندگان
چکیده
منابع مشابه
an introduction to rna interference (rnai)
the discovery of rna interference (rnai) in recent years has revolutionized biological and medical research. rnai is the sequence-specific gene silencing triggered by double-stranded rna (dsrna). until a couple of years ago only biologists working with caenorhabditis elegans and plants could use rnai technology to gain insight into gene function……
متن کاملEnhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase
Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...
متن کاملRNA Interference (RNAi) Screening in Drosophila
In the last decade, RNA interference (RNAi), a cellular mechanism that uses RNA-guided degradation of messenger RNA transcripts, has had an important impact on identifying and characterizing gene function. First discovered in Caenorhabditis elegans, RNAi can be used to silence the expression of genes through introduction of exogenous double-stranded RNA into cells. In Drosophila, RNAi has been ...
متن کاملRNA Interference: Systemic RNAi SIDes with Endosomes
Systemic RNAi, the intercellular spreading of RNAi silencing, requires SID-1 and SID-3 to import silencing signals in Caenorhabditis elegans. How are these signals exported? SID-5, an endosome-associated protein, is a candidate for the job.
متن کاملMedical applications of RNA interference (RNAi)
During its long and rich history, medical sciences have undergone several changes of paradigms. In this quest to develop more potent drugs, less invasive surgical techniques and more sophisticated diagnostic approaches (among other goals) medicine has contributed decisively to our well being and longevity. Some of these major accomplishments, which altered the course of humanity are: the develo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cancer Biology & Therapy
سال: 2003
ISSN: 1538-4047,1555-8576
DOI: 10.4161/cbt.2.2.358